The core premise and narrative remain traditional genre fare focused on family legacy, hunting, and personal closure rather than identity politics. However, the show deliberately foregrounds progressive casting and representation through main cast member Carlos Cervantes, a non-binary demon hunter played by non-binary actor Jojo Fleites, who receives substantial screen time including personal arcs and stakeouts.

Creators including Jensen Ackles explicitly described the inclusion of non-binary and diverse characters as a mission to make the series more inclusive across genders, ethnicities, and sexualities, with Fleites highlighting the space given to LGBTQ+ perspectives. Supporting roles add further diversity via actors like Nida Khurshid as Latika and Demetria McKinney as Ada. Audience reactions included Reddit critiques labeling the show "woke" for its tone and casting choices, though no source-material character alterations occurred for the leads.