The story opens with Chucky and Tiffany debating whether to name their offspring Glen or Glenda after observing the absence of genitals, and the child directly poses the question of being both. Glen is depicted as pacifist while Glenda embodies violence, with the plot resolving through voodoo soul-splitting that produces separate male and female human twins.

This identity conflict drives the emotional core alongside the horror elements and Hollywood satire, explicitly nodding to Ed Wood's Glen or Glenda. Creator Don Mancini, writing and directing, deliberately foregrounds these queer themes as the film's premise, using a John Waters cameo and explicit dialogue to frame the doll's confusion as a gender identity issue. The resolution affirms the child's desire to 'be both' through supernatural means, turning the movie into a vehicle for progressive messaging on non-binary identity. This approach intrudes on the franchise's established slasher entertainment, subordinating scares and satire to ideological affirmation and resulting in a compromised, agenda-driven experience.