Harley Quinn is explicitly bisexual, King Shark is confirmed queer/bisexual in the Arkhamverse tie-in comic and prior media, Captain Boomerang is framed as pansexual, and DLC introduces Mrs. Freeze as a lesbian with an Asian redesign. Pride-themed outfits, emotes, and banner flags for all playable characters were released as free content, alongside an in-world LGBTQ+ flag spotted in Metropolis gameplay footage.

Sweet Baby Inc. provided late-stage scriptwriting for audio logs, NPC dialogue, and advertisements, aligning with their known DEI consulting role. The core premise of anti-hero misfits killing the Justice League includes portrayals of traditional heroes as brainwashed threats and Squad members as sympathetic victims, drawing criticism for undermining source material.

Reception shows a clear critic-user gap (Metacritic critics ~60, users ~3.5/10) and widespread player backlash labeling the game 'woke' over the flag, Pride marketing, and perceived disrespect to characters like Batman. These elements influence casting, subplots, and marketing without fully redefining the live-service shooter premise, placing the score in the significant but not foundational range.